Crossreactivity of boar sperm monoclonal antibodies with human spermatozoa
نویسندگان
چکیده
منابع مشابه
Monoclonal antibodies to human sperm antigens.
To elucidate the molecular nature of human sperm autoantigens, attempts were made to raise monoclonal antibodies against these antigens, by hybridoma techniques. After successive immunizations with the particulate fractions of human sperm extract in BALB/c mice, the spleen cells were fused with P3-X63-Ag8 myeloma cells. Several clones and their subclones were obtained and shown by microplate ra...
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As part of our continuous effort to elucidate the biochemical and immunological nature of human sperm surface antigens, monoclonal antibodies to human spermatozoa were generated by improved hybridoma techniques. Following immunizations with the membrane fraction of human spermatozoa and cell fusions, hybrid cells were cultured in a semi-solid HAT-selection medium to maximize the number of monoc...
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Alpha-fetoprotein (AFP), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. Monoclonal anti-AFP antibodies are essential reagents in developing appropriate techniques for measurement of this protein. In this study, in order to produce anti-AFP monoclonal a...
متن کاملProduction and immunolocalization of monoclonal antibodies against human sperm
In order to help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm surface antigens. The IgG fraction of serum from a donor with a spontaneous high titer of IgG-positive antisperm reactivity (as determined by immunobead binding) was purified by ammonium sulfate precipitation. This IgG preparation w...
متن کاملA panel of monoclonal antibodies against human sperm.
To help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm antigens. The immunoglobulin G (IgG) fraction of serum from a male donor with a spontaneous high titer of IgG-positive antisperm reactivity (as determined by immunobead binding) was purified by ammonium sulfate precipitation. This IgG prepar...
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ژورنال
عنوان ژورنال: African Journal of Biomedical Research
سال: 2010
ISSN: 1119-5096
DOI: 10.4314/ajbr.v7i1.54065